Journal: Current biology : CB
Article Title: Centrifugal Displacement of Nuclei Reveals Multiple LINC Complex Mechanisms for Homeostatic Nuclear Positioning
doi: 10.1016/j.cub.2017.08.073
Figure Lengend Snippet: Key Resources Table
Article Snippet: Western blots were developed with IRDye® 680 and IRDye® 800CW secondary antibodies raised in donkey (LI-COR Biosciences) and imaged with Odyssey. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SUN2 Abcam CAT # ab87036 Mouse monoclonal anti-p150[Glued] BD Biosciences CAT # 610473 Mouse monoclonal anti-GM130 BD Biosciences CAT # 610822 Mouse monoclonal anti-pericentrin BD biosciences CAT # 611814 Rabbit polyclonal anti-pericentrin Covance CAT # PRB-432C Rat monoclonal anti-Tyr tubulin European Collection of Animal Cell Cultures YL1/2 Rabbit polyclonal anti-SUN1 Gift of Dr. S. Shackleton, U. of Leicester NA Mouse monoclonal anti-DIC Gift of Dr. R. B. Vallee, Columbia U NA Mouse monoclonal anti-KLC 63-90 Gift of Dr. S. T. Brady, U. of Illinois at Chicago NA Donkey anti-chicken Alexa 488 Jackson ImmunoResearch Laboratories CAT # 703-545-155 Donkey anti-mouse Alexa 488 Jackson ImmunoResearch Laboratories CAT # 715-545-151 Donkey anti-mouse Cy3 Jackson ImmunoResearch Laboratories CAT # 715-165-151 Donkey anti-rabbit Cy2 Jackson ImmunoResearch Laboratories CAT # 711-225-152 Donkey anti-rabbit Cy3 Jackson ImmunoResearch Laboratories CAT # 711-165-152 Donkey anti-rat Alexa 647 Jackson ImmunoResearch Laboratories CAT # 712-605-153 Donkey anti-mouse IRDye® 680 LI-COR Biosciences P/N # 925-68072 Donkey anti-rabbit IRDye® 680 LI-COR Biosciences P/N # 925-68073 Donkey anti-mouse IRDye® 800CW LI-COR Biosciences P/N # 925-32212 Donkey+A20 anti-rabbit IRDye® 800CW LI-COR Biosciences P/N # 925-32213 Rabbit polyclonal anti-nepsin 2G [11] S2-CH Mouse monoclonal anti-KHC Millipore MAB1614.
Techniques: Recombinant, Mutagenesis, Immunoprecipitation, Transfection, Construct, Software